nicking reaction catalysed with the MobA89K and MobC89K proteins. The cleaved strand is then unwound via the helicase and shipped to the 89K-encoded GI-style T4SS machinery. The VirB1-89K part is thought to work as a lytic transglycosylase, regionally opening the peptidoglycan with contributions from Another aspect(s) over the assembly with the tra